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KMID : 0861020220370060037
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Korea Journal of Herbology
2022 Volume.37 No. 6 p.37 ~ p.44
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Chondro-protective and Anti-inflammatory Effects of Sorbi Commixtae Fructus Extract
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Park Dong-Jun
Lee Hong-Gu
Ko Chung-Ho
Park Hyoung-Kook
Jin Mu-Hyun
Cho Ho-Song
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Abstract
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Objectives : From this study, we sight to identify chondro-protective and anti-inflammatory effects of Sorbi Commixtae
Fructus extract and its compound, chlorogenic acid.
Methods : Sorbi Commixtae Fructus were extracted by 50% ethanol. And chlorogenic acid in Sorbi Commixtae Fructus
50% extract was quantified by high performance liquid chromatography (HPLC). To investigate chondro-protective
effects, we treated Sorbi Commixtae Fructus 50% ethanol extract and chlorogenic acid in TNFα-activated ATDC5
murine chondrogenic cells. After 24 hours, protein level of matrix metalloproteinase-3 (MMP3) and mRNA level of
matrix metalloproteinase-13 (MMP13) were measured by using ELISA or reverse transcription PCR, respectively.
To examine anti-inflammatory effects, we treated Sorbi Commixtae Fructus 50% ethanol extract and chlorogenic
acid in LPS-induced RAW 264.7 murine macrophages. We measured the level of inflammatory mediators, such as Prostaglandin E2 (PGE2), Interleukin-6 (IL6) by ELISA and nitric oxide (NO) by Griess reagent assay.
Results : A concentration of chlorogenic acid in Sorbi Commixtae Fructus 50% ethanol extract was 3.9 mg/g. Sorbi
Commixtae Fructus 50% ethanol extract and chlorogenic acid attenuated protein level of MMP3 and mRNA level of
MMP13 in TNFα-activated ATDC5 cells. Sorbi commixtae Fructus 50% ethanol extract inhibited the level of PGE2, IL6
and NO in LPS-activated RAW 264.7 cells in dose dependent manner, but chlorogenic acid has no anti-inflammatory
effects.
Conclusions : These findings demonstrated that Sorbi Commixtae Fructus 50% ethanol extract has chondro-protective
and anti-inflammatory effects showing possible therapeutics to ease the symptoms related with osteoarthritis. 1)
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KEYWORD
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Sorbi Commixtae Fructus, Chlorogenic acid, Chondro-protection, Anti-inflammation
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